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ABSTRACT The BacA protein is essential for the long-term survival of Sinorhizobium meliloti and Brucella abortus within acidic compartments in plant and animal cells, respectively. Since both the S. meliloti and B. abortus bacA mutants have an increased resistance to bleomycin, it was hypothesized that BacA was a transporter of bleomycin and bleomycin-like compounds into the bacterial cell.

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Purified accessory hemicellulase synergy studies have been conducted for low severity, hot-water pretreated corn stover (Selig et al., 2008b; Knoshaug et al., 2008). It was found that glucan conversions to cellobiose (enzyme loading = 15 and 50 mg CBH I/g glucan) by CBH I increased by 13–84% in the presence of a suitable acetyl xylan and/or feruloyl esterase (enzyme loading = 2.5 mg/g glucan).

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When Rhizobium etli CE3 was grown in the presence of Phaseolus vulgaris seed extracts containing anthocyanins, its lipopolysaccharide (LPS) sugar composition was changed in two ways: greatly decreased content of what is normally the terminal residue of the LPS, di-O-methylfucose, and a doubling of the 2-O-methylation of other fucose residues in the LPS O antigen.

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7. Yersinia enterocolitica 62 S at 40 C. 8. Salmonella (vphimurium Ra mutant (his 386). 9. teus and Serratia [23]. The presence, as well as the Salmonella (vphtmurtum Rb 2 mutant (TV 166). 10. Salmonella content, of 4-AA envisages these

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ABSTRACT The BacA protein is essential for the long-term survival of Sinorhizobium meliloti and Brucella abortus within acidic compartments in plant and animal cells, respectively. Since both the S. meliloti and B. abortus bacA mutants have an increased resistance to bleomycin, it was hypothesized that BacA was a transporter of bleomycin and bleomycin-like compounds into the bacterial cell.

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1/2/2015 · Gamma ray-induced in vitro mutagenesis and selection for salt (NaCl) tolerance were investigated in sugarcane (Saccharum officinarum L.).Embryogenic callus cultures were irradiated (10 to 80 Gy) and subjected to in vitro selection by exposure of irradiated callus to NaCl (0, 50, 100, 150, 200, and 250 mmol L − 1).).

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Abstract. Seed coat mucilages are mainly polysaccharides covering the outer layer of the seeds to facilitate seed hydration and germination, thereby improving seedling emergence and reducing seedling mortality. Four types of polysaccharides are found in mucilages …

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The BacA protein is essential for the long-term survival of Sinorhizobium meliloti and Brucella abortus within acidic compartments in plant and animal cells, respectively. Since both the S. meliloti and B. abortus bacA mutants have an increased resistance to bleomycin, it was hypothesized that BacA was a transporter of bleomycin and bleomycin-like compounds into the bacterial cell.

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Deficiency of a Sinorhizobium meliloti bacA Mutant in …

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1/2/2015 · The explant material was washed first in tap water and then for 5 min in sterile distilled water at least three times. Surface decontamination was performed with 80% ethanol (v/v) for 5 min and mercury chloride (0.1% w/v) for 4–5 min, followed by three washes with sterile distilled water …

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The SDS lysis method was performed as described previously, except the samples were boiled for 10 min and then treated with proteinase K (final concentration of 0.6 mg/ml) at 60°C for 1 h. The LPS samples were suspended in SDS sample buffer (50 μl for the SDS lysis method and 20 μl for the hot water …

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